Ponsford, Amy H.; Ryan, Thomas A.; Raimondi, Andrea; Cocucci, Emanuele; Wycislo, Susanne A.; Frohlich, Florian; Swan, Laura E.; Stagi, Massimiliano published the artcile< Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor>, Safety of 9-(6-Aminopyridin-3-yl)-1-(3-(trifluoromethyl)phenyl)benzo[h][1,6]naphthyridin-2(1H)-one, the main research area is chloroquine fluorescence microscopy lysosome MTOR protein pH Vtype ATPase; Chloroquine; MTOR protein; V-type ATPase; fluorescence microscopy; lysosomes; pH.
Disorders of lysosomal physiol. have increasingly been found to underlie the pathol. of a rapidly growing cast of neurodevelopmental disorders and sporadic diseases of aging. One cardinal aspect of lysosomal (dys)function is lysosomal acidification in which defects trigger lysosomal stress signaling and defects in proteolytic capacity. We have developed a genetically encoded ratiometric probe to measure lysosomal pH coupled with a purification tag to efficiently purify lysosomes for both proteomic and in vitro evaluation of their function. Using our probe, we showed that lysosomal pH is remarkably stable over a period of days in a variety of cell types. Addnl., this probe can be used to determine that lysosomal stress signaling via TFEB is uncoupled from gross changes in lysosomal pH. Finally, we demonstrated that while overexpression of ARL8B GTPase causes striking alkalinization of peripheral lysosomes in HEK293 T cells, peripheral lysosomes per se are no less acidic than juxtanuclear lysosomes in our cell lines.
Autophagy published new progress about Acidification. 1223001-51-1 belongs to class naphthyridine, and the molecular formula is C24H15F3N4O, Safety of 9-(6-Aminopyridin-3-yl)-1-(3-(trifluoromethyl)phenyl)benzo[h][1,6]naphthyridin-2(1H)-one.
Referemce:
1,8-Naphthyridine – Wikipedia,
1,8-Naphthyridine | C8H6N2 – PubChem