Chen, Mengchun; Jiang, Qi; Zhang, Mingyao; Chen, Sailing; Lou, Junsheng; Chen, Yijie; Wang, Fang; Wang, Rongyue published the artcile< Establishment of quantitative methodology for sophoridine analysis and determination of its pharmacokinetics and bioavailability in rat>, Reference of 6882-68-4, the main research area is sophoridine detection blood circulation pharmacokinetics bioavailability rat; Sophoridine; UPLC–MS/MS; bioavailability; pharmacokinetics; rat plasma.
The aim of this study is to develop a rapid and sensitive UPLC-MS/MS approach to determine the sophoridine (SOP) level in rat plasma and the pharmacokinetics of the substance. Sophoridine is used as an anti-inflammatory, anti-virus, anti-microbial, and anti-tumor alkaloid. It is essential to explore specific detection methods for the quant. anal. of SOP in the blood circulation. The rat plasma samples were prepared by one-step protein precipitation with acetonitrile. Subsequently, the samples were separated by chromatog. using a UPLC BEH C18 reversed-phase with an initial mobile phase of methanol and 0.1% formic acid aqueous solution The gradient elution was performed at a fixed flow rate of 0.4 mL/min, and multiple reaction monitoring (MRM) mode with an electrospray pos. ionization source was employed to detect the transitions of m/z 249.1 → 84.2 for SOP and m/z 264.3 → 69.8 for dendrobine (IS). The entire process required 3.5 min for each sample. A linear correlation was established over the range of 2-2000 ng/mL (r2≥0.9954) for SOP in rat plasma with a lower limit of quantification (LLOQ) at 2 ng/mL. The range of accuracy was tested between 94.90% and 100.80%, and the relative standard deviations (RSDs) toward both intra- and inter-day precision were <10%. Thus, this method was successfully applied to a pharmacokinetic study, and the subsequent results demonstrated a low absolute bioavailability of 2.32%. The present study established a reliable method that quantified the SOP concentration in rat plasma after administering a dose of 2 mg/kg i.v. or 20 mg/kg orally. Drug Development and Industrial Pharmacy published new progress about Alkaloids Role: ANT (Analyte), BSU (Biological Study, Unclassified), THU (Therapeutic Use), ANST (Analytical Study), BIOL (Biological Study), USES (Uses). 6882-68-4 belongs to class naphthyridine, and the molecular formula is C15H24N2O, Reference of 6882-68-4.
Referemce:
1,8-Naphthyridine – Wikipedia,
1,8-Naphthyridine | C8H6N2 – PubChem