Wang, Qingxiang published the artcileA sensitive DNA biosensor based on a facile sulfamide coupling reaction for capture probe immobilization, Computed Properties of 116-63-2, the publication is Analytica Chimica Acta (2013), 158-164, database is CAplus and MEDLINE.
A novel DNA biosensor was fabricated through a facile sulfamide coupling reaction. First, the versatile sulfonic dye mol. of 1-amino-2-naphthol-4-sulfonate (AN-SO3–) was electrodeposited on the surface of a glassy carbon electrode (GCE) to form a steady and ordered AN-SO3– layer. Then the amino-terminated capture probe was covalently grafted to the surface of SO3–-AN deposited GCE through the sulfamide coupling reaction between the amino groups in the probe DNA and the sulfonic groups in the AN-SO3–. The step-by-step modification process was characterized by electrochem. and attenuated total reflectance Fourier transform IR (ATR-FTIR) spectroscopy. Using Ru(NH3)63+ as probe, the probe d. and the hybridization efficiency of the biosensor were determined to be 3.18 × 1013 strands cm-2 and 86.5%, resp. The hybridization performance of the biosensor was examined by differential pulse voltammetry using Co(phen)33+/2+ (phen = 1,10-phenanthroline) as the indicator. The selectivity experiments showed that the biosensor presented distinguishable response after hybridization with the three-base mismatched, non-complementary and complementary sequences. Under the optimal conditions, the oxidation peak currents of Co(phen)33+/2+ increased linearly with the logarithm values of the concentration of the complementary sequences in the range from 1.0 × 10-13 M to 1.0 × 10-8 M with a regression coefficient of 0.9961. The detection limit was estimated to be 7.2 × 10-14 M based on 3σ.
Analytica Chimica Acta published new progress about 116-63-2. 116-63-2 belongs to naphthyridine, auxiliary class Sulfonic acid,Amine,Naphthalene,Alcohol,Organic Pigment, name is 4-Amino-3-hydroxynaphthalene-1-sulfonic acid, and the molecular formula is C17H14N2O2, Computed Properties of 116-63-2.
Referemce:
https://en.wikipedia.org/wiki/1,8-Naphthyridine,
1,8-Naphthyridine | C8H6N2 – PubChem